Human CD30 Ligand/TNFSF8 Protein, Accession#: P32971, CAS#: 3202-706-815; ChemWhat Code: 1413083
Overview
| Product Description | Recombinant Human CD30 Ligand/TNFSF8 Protein is expressed from HEK293 with mFc (IgG2a) tag at the N-terminal.It contains Gln63-Asp234. |
| Biotinylated |
Specification
| Target | CD30 Ligand/TNFSF8 |
| Synonyms | CD153; CD30 Ligand; CD30L; CD30-L; CD30LGMGC138144; TNFSF8; CD30LG |
| Gene/Protein ID (Accession) | P32971 |
| Species | Human |
| Exact Sequence | Gln63-Asp234 |
| Molecular Weight | The protein has a predicted MW of 46.2 kDa. Due to glycosylation, the protein migrates to 62-68 kDa based on Tris-Bis PAGE result. |
| Activity | Immobilized Human CD30, His Tag at 0.2ug/ml (100ul/well) on the plate. Dose response curve for Human CD30 Ligand, mFc (IgG2a) Tag with the EC50 of 31.9ng/ml determined by ELISA. See testing image for detail. |
| Product Tag | N-mFc |
| Express System | HEK293 |
| Endotoxin | Less than 1EU per ug by the LAL method. |
Shipping, Reconstitution & Storage
| Form | Lyophilized |
| Shipping | Shipped at ambient temperature. |
| Formulation | Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| Reconstitution | Centrifuge tubes before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Stability and Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
Validation Spectrums
| Spectrums |  |
Buy Reagent | |
| ChemWhat 1413083 | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC; |
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