Human CD73/NT5E Protein, Accession#: P21589, CAS#: 3202-706-2053; ChemWhat Code: 1414547
Overview
| Product Description | Recombinant Human CD73/NT5E Protein is expressed from HEK293 with hFC tag at the C-terminal.It contains Trp27-Lys547. |
| Biotinylated |
Specification
| Target | CD73/NT5E |
| Synonyms | CD73; NT5E; NTE; 5′ nucleotidase (CD73); 5′-NT; 5-NT; E5NT; EC 3.1.3.5; eN; eNT; NT; NT55′-nucleotidase; NT5; NTE; CALJA |
| Gene/Protein ID (Accession) | P21589 |
| Species | Human |
| Exact Sequence | Trp27-Lys547 |
| Molecular Weight | The protein has a predicted MW of 84.5 kDa. Due to glycosylation, the protein migrates to 85-100 kDa based on Tris-Bis PAGE result. |
| Activity | Immobilized Human CD73, hFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-CD73 Antibody, hFc Tag with the EC50 of 22.6ng/ml determined by ELISA (QC Test). See testing image for detail. |
| Product Tag | C-hFc |
| Express System | HEK293 |
| Endotoxin | Less than 1EU per ug by the LAL method. |
Shipping, Reconstitution & Storage
| Form | Lyophilized |
| Shipping | Shipped at ambient temperature. |
| Formulation | Lyophilized from 0.22um filtered solution in 20mM Tris, 120mM NaCl (pH 7.5). Normally 8% trehalose is added as protectant before lyophilization. |
| Reconstitution | Centrifuge tubes before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Stability and Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
Validation Spectrums
| Spectrums |  |
Buy Reagent | |
| ChemWhat 1414547 | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
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Other Suppliers | |
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