Human CDCP1 Protein, Accession#: Q9H5V8, CAS#: 3202-706-903; ChemWhat Code: 1413237

Overview Specification
Shipping, Reconstitution & Storage Validation Spectrums

Overview

Product Description Recombinant Human CDCP1 Protein is expressed from HEK293 with His tag at the C-terminal.It contains Phe30-Leu666.
Biotinylated

Specification

Target CDCP1
Synonyms CDCP1; CD318; SIMA135; TRASK; UNQ2486; PRO5773
Gene/Protein ID (Accession) Q9H5V8
Species Human
Exact Sequence Phe30-Leu666
Molecular Weight The protein has a predicted MW of 72.8 kDa. Due to glycosylation, the protein migrates to 80-100 kDa based on Tris-Bis PAGE result.
Activity Immobilized Human CDCP1 at 2ug/ml (100ul/Well). Dose response curve for Anti-CDCP1 Antibody, hFc Tag with the EC50 of 8.0ng/ml determined by ELISA (QC Test). The affinity constant of 0.18nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag C-His
Express System HEK293
Endotoxin Less than 1EU per ug by the LAL method.

Shipping, Reconstitution & Storage

Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability and Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

Validation Spectrums

Spectrums

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ChemWhat 1413237> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
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