Human/Cynomolgus/Rhesus macaque CD28 Protein, Accession#: P10747, CAS#: 3202-706-1015; ChemWhat Code: 1413415
Overview
| Product Description | Recombinant Human/Cynomolgus/Rhesus macaque CD28 Protein is expressed from HEK293 with mFc (IgG2a) tag at the N-terminal.It contains Asn19-Pro152. |
| Biotinylated |
Specification
| Target | CD28 |
| Synonyms | CD28 antigen (Tp44); CD28 antigen; CD28 molecule; CD28; MGC138290; T-cell-specific surface glycoprotein CD28; Tp44 |
| Gene/Protein ID (Accession) | P10747 |
| Species | Human/Cynomolgus/Rhesus macaque |
| Exact Sequence | Asn19-Pro152 |
| Molecular Weight | The protein has a predicted MW of 41.9 kDa. Due to glycosylation, the protein migrates to 65-68 kDa based on Tris-Bis PAGE result. |
| Activity | Immobilized Human/Cynomolgus/Rhesus macaque CD28, mFc (IgG2a) Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-CD28 Antibody, hFc Tag with the EC50 14.5ng/ml determined by ELISA. See testing image for detail. |
| Product Tag | N-mFc |
| Express System | HEK293 |
| Endotoxin | Less than 1EU per ug by the LAL method. |
Shipping, Reconstitution & Storage
| Form | Lyophilized |
| Shipping | Shipped at ambient temperature. |
| Formulation | Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| Reconstitution | Centrifuge tubes before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Stability and Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
Validation Spectrums
| Spectrums |  |
Buy Reagent | |
| ChemWhat 1413415 | > 95% as determined by Tris-Bis PAGE |
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