Human DKK1 C terminal Domain Protein, Accession#: O94907, CAS#: 3202-706-515; ChemWhat Code: 1412628
Overview
| Product Description | Recombinant Human DKK1 C terminal Domain Protein is expressed from HEK293 with hFc tag and Avi tag at the C-terminal.It contains Met178-His266. |
| Biotinylated |
Specification
| Target | DKK1 C terminal Domain |
| Synonyms | |
| Gene/Protein ID (Accession) | O94907 |
| Species | Human |
| Exact Sequence | Met178-His266 |
| Molecular Weight | The protein has a predicted MW of 38.69 kDa. Due to glycosylation, the protein migrates to 45-55 kDa based on Tris-Bis PAGE result. |
| Activity | Immobilized Human DKK1 C terminal Domain, hFc Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Anti-DKK1 Antibody, hFc Tag with the EC50 of 26.7ng/ml determined by ELISA. See testing image for detail. |
| Product Tag | C-hFc-Avi |
| Express System | HEK293 |
| Endotoxin | Less than 1EU per ug by the LAL method. |
Shipping, Reconstitution & Storage
| Form | Lyophilized |
| Shipping | Shipped at ambient temperature. |
| Formulation | Lyophilized from 0.22um filtered solution in 20mM NaAc,150mM NaCl (pH 5.0). Normally 8% trehalose is added as protectant before lyophilization. |
| Reconstitution | Centrifuge tubes before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in 20mM NaAc,150mM NaCl (pH 5.0). |
| Stability and Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
Validation Spectrums
| Spectrums |  |
Buy Reagent | |
| ChemWhat 1412628 | > 95% as determined by Tris-Bis PAGE |
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