Human EpCAM/TROP1 Protein, Accession#: P16422, CAS#: 3202-706-1192; ChemWhat Code: 1413655
Overview
| Product Description | Recombinant Human EpCAM/TROP1 Protein is expressed from HEK293 with hFc tag at the C-terminal.It contains Gln24-Lys245. |
| Biotinylated |
Specification
| Target | EpCAM/TROP1 |
| Synonyms | CD326; EGP; EGP-2; EGP314; EGP40; EpCAM; 17-1A; 323/A3; ACSTD1; DIAR5; GA733-2; gp40; HNPCC8; KS1/4; KSAHEA125; M1S2; M4S1; M4S1Ly74; MIC18MH99; MOC31; TACST-1; TACSTD1; TROP1; ESA; KS1,4; KSA; MIC18; MK1 |
| Gene/Protein ID (Accession) | P16422 |
| Species | Human |
| Exact Sequence | Gln24-Lys245 |
| Molecular Weight | The protein has a predicted MW of 54.2 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Tris-Bis PAGE result. |
| Activity | Immobilized Human EpCAM, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-EpCAM Antibody, hFc Tag with the EC50 of 18.9ng/ml determined by ELISA. See testing image for detail. |
| Product Tag | C-hFc |
| Express System | HEK293 |
| Endotoxin | Less than 1EU per ug by the LAL method. |
Shipping, Reconstitution & Storage
| Form | Lyophilized |
| Shipping | Shipped at ambient temperature. |
| Formulation | Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. |
| Reconstitution | Centrifuge tubes before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Stability and Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
Validation Spectrums
| Spectrums |  |
Buy Reagent | |
| ChemWhat 1413655 | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
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