Human Fc gamma RIIIA/CD16a (V176) Protein, Accession#: AAH17865, CAS#: 3202-706-1735; ChemWhat Code: 1414228
Overview
| Product Description | Recombinant Human Fc gamma RIIIA/CD16a (V176) Protein is expressed from HEK293 with His tag and Avi tag at the C-terminal.It contains Gly17-Gln208(V176). |
| Biotinylated |
Specification
| Target | Fc gamma RIIIA/CD16a (V176) |
| Synonyms | CD16FCRIIIA; Fc fragment of IgG; Fc gamma RIIIA; Fc-gamma RIII-alpha; FCGR3; FCGR3A; FCGRIII; FcgRIIIA; FcR-10; CD16A; FCG3; IGFR3; FcRIIIa; FcRIII; CD16; IMD20 |
| Gene/Protein ID (Accession) | AAH17865 |
| Species | Human |
| Exact Sequence | Gly17-Gln208(V176) |
| Molecular Weight | The protein has a predicted MW of 24.7 kDa. Due to glycosylation, the protein migrates to 50-55 kDa based on Tris-Bis PAGE result. |
| Activity | Immobilized Human Fc gamma RIIIA (V176) , His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Anti-Fc gamma RIIIA Antibody, hFc Tag with the EC50 of 23.3ng/ml determined by ELISA (QC test). The affinity constant of 67.3nM as determined in SPR assay (Biacore T200). See testing image for detail. |
| Product Tag | C-His-Avi |
| Express System | HEK293 |
| Endotoxin | Less than 1EU per ug by the LAL method. |
Shipping, Reconstitution & Storage
| Form | Lyophilized |
| Shipping | Shipped at ambient temperature. |
| Formulation | Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. |
| Reconstitution | Centrifuge tubes before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Stability and Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
Validation Spectrums
| Spectrums |  |
Buy Reagent | |
| ChemWhat 1414228 | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
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