Human FcRn Protein, Accession#: P55899, CAS#: 3202-706-1376; ChemWhat Code: 1413871
Overview
| Product Description | Recombinant Human FcRn Protein is expressed from HEK293 with His tag at the C-terminal.It contains Ala24-Ser297(FCGRT) & Ile21-Met119(B2M). |
| Biotinylated |
Specification
| Target | FcRn |
| Synonyms | FcRn alpha chain; FCGRT; FCRN; FCGRT&B2M |
| Gene/Protein ID (Accession) | P55899 |
| Species | Human |
| Exact Sequence | Ala24-Ser297(FCGRT) & Ile21-Met119(B2M) |
| Molecular Weight | The protein has a predicted MW of 31.5 kDa (FCGRT) & 11.7 kDa (B2M). Due to glycosylation, the protein migrates to 35-40 kDa (FCGRT) & 13-14 kDa (B2M) based on Tris-Bis PAGE result. |
| Activity | Immobilized Human FcRn, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-FcRn Antibody, hFc Tag with the EC50 of 41.1ng/ml determined by ELISA (QC Test). The affinity constant of 0.28 uM as determined in SPR assay (Biacore T200). See testing image for detail. |
| Product Tag | C-His |
| Express System | HEK293 |
| Endotoxin | Less than 1EU per ug by the LAL method. |
Shipping, Reconstitution & Storage
| Form | Lyophilized |
| Shipping | Shipped at ambient temperature. |
| Formulation | Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| Reconstitution | Centrifuge tubes before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Stability and Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
Validation Spectrums
| Spectrums |  |
Buy Reagent | |
| ChemWhat 1413871 | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
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