Human Galectin 3 Protein, Accession#: P17931, CAS#: 3202-706-1359; ChemWhat Code: 1413853
Overview
| Product Description | Recombinant Human Galectin 3 Protein is expressed from HEK293 with His tag at the N-terminal.It contains Ala2-Ile250. |
| Biotinylated |
Specification
| Target | Galectin 3 |
| Synonyms | AGE-R3; CBP35; GAL3; Gal-3; galactin-3; GALBPCBP35; Galectin3; GALIG; L29; L31; Lectin L-29; LGALS2; LGALS3; Mac-2; MAC2GAL3 |
| Gene/Protein ID (Accession) | P17931 |
| Species | Human |
| Exact Sequence | Ala2-Ile250 |
| Molecular Weight | The protein has a predicted MW of 27.1 kDa. Due to glycosylation, the protein migrates to 35-45 kDa based on Tris-Bis PAGE result. |
| Activity | Immobilized Human Galectin 3, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-Galectin 3 Antibody, hFc Tag with the EC50 of 17.3ng/ml determined by ELISA (QC Test). The affinity constant of 33.30 nM as determined in SPR assay (Biacore T200). See testing image for detail. |
| Product Tag | N-His |
| Express System | HEK293 |
| Endotoxin | Less than 1EU per ug by the LAL method. |
Shipping, Reconstitution & Storage
| Form | Lyophilized |
| Shipping | Shipped at ambient temperature. |
| Formulation | Lyophilized from 0.22um filtered solution in 50mM HEPES, 150mM NaCl, 5mM EDTA, 2mM TCEP (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| Reconstitution | Centrifuge tubes before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Stability and Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
Validation Spectrums
| Spectrums |  |
Buy Reagent | |
| ChemWhat 1413853 | > 95% as determined by Tris-Bis PAGE |
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