Human GITR Ligand/TNFSF18 Trimer Protein, Accession#: Q9UNG2, CAS#: 3202-706-1389; ChemWhat Code: 1413889

Overview Specification
Shipping, Reconstitution & Storage Validation Spectrums

Overview

Product Description Recombinant Human GITR Ligand/TNFSF18 Trimer Protein is expressed from HEK293 with His tag and flag tag at the N-terminal.It contains Gln50-Ser177.
Biotinylated

Specification

Target GITR Ligand/TNFSF18 Trimer
Synonyms TNFSF18; AITRL; TL6; GITRL; GITR Ligand
Gene/Protein ID (Accession) Q9UNG2
Species Human
Exact Sequence Gln50-Ser177
Molecular Weight The protein has a predicted MW of 47.5 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on Tris-Bis PAGE result.
Activity Immobilized Human GITR Ligand Trimer, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human GITR, hFc Tag with the EC50 of 0.44ug/ml determined by ELISA. See testing image for detail.
Product Tag N-His-Flag
Express System HEK293
Endotoxin Less than 1EU per ug by the LAL method.

Shipping, Reconstitution & Storage

Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability and Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

Validation Spectrums

Spectrums

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ChemWhat 1413889> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
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