Human GITR Ligand/TNFSF18 Trimer Protein, Accession#: Q9UNG2, CAS#: 3202-706-1389; ChemWhat Code: 1413889
Overview
Product Description | Recombinant Human GITR Ligand/TNFSF18 Trimer Protein is expressed from HEK293 with His tag and flag tag at the N-terminal.It contains Gln50-Ser177. |
Biotinylated |
Specification
Target | GITR Ligand/TNFSF18 Trimer |
Synonyms | TNFSF18; AITRL; TL6; GITRL; GITR Ligand |
Gene/Protein ID (Accession) | Q9UNG2 |
Species | Human |
Exact Sequence | Gln50-Ser177 |
Molecular Weight | The protein has a predicted MW of 47.5 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on Tris-Bis PAGE result. |
Activity | Immobilized Human GITR Ligand Trimer, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human GITR, hFc Tag with the EC50 of 0.44ug/ml determined by ELISA. See testing image for detail. |
Product Tag | N-His-Flag |
Express System | HEK293 |
Endotoxin | Less than 1EU per ug by the LAL method. |
Shipping, Reconstitution & Storage
Form | Lyophilized |
Shipping | Shipped at ambient temperature. |
Formulation | Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution | Centrifuge tubes before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability and Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
Validation Spectrums
Spectrums | |
Buy Reagent | |
ChemWhat 1413889 | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
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