Human GM-CSF Protein, Accession#: P04141, CAS#: 3202-706-909; ChemWhat Code: 1413245
Overview
| Product Description | Recombinant Human GM-CSF Protein is expressed from E.coli without tag.It contains Ala18-Glu144. |
| Biotinylated |
Specification
| Target | GM-CSF |
| Synonyms | CSF; CSF2; GMCSF; GM-CSF; MGC131935; MGC138897 |
| Gene/Protein ID (Accession) | P04141 |
| Species | Human |
| Exact Sequence | Ala18-Glu144 |
| Molecular Weight | The protein has a predicted MW of 14.6 kDa same as Tris-Bis PAGE result. |
| Activity | Immobilized Human GM-CSF at 2ug/ml (100ul/Well) on the plate. Dose response curve for Human GM-CSF R alpha, hFc Tag with the EC50 of 16.9ng/ml determined by ELISA (QC Test). The affinity constant of 0.79nM as determined in SPR assay (Biacore T200). See testing image for detail. |
| Product Tag | No Tag |
| Express System | E.coli |
| Endotoxin | Less than 1EU per ug by the LAL method. |
Shipping, Reconstitution & Storage
| Form | Lyophilized |
| Shipping | Shipped at ambient temperature. |
| Formulation | Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. |
| Reconstitution | Centrifuge tubes before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Stability and Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
Validation Spectrums
| Spectrums |  |
Buy Reagent | |
| ChemWhat 1413245 | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
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