Human GUCY2C/Guanylyl cyclase C Protein, Accession#: P25092, CAS#: 3202-706-382; ChemWhat Code: 1412381
Overview
| Product Description | Recombinant Human GUCY2C/Guanylyl cyclase C Protein is expressed from HEK293 with hFc tag at the C-terminal.It contains Ser24-Gln430. |
| Biotinylated |
Specification
| Target | GUCY2C/Guanylyl cyclase C |
| Synonyms | Guanylyl cyclase C; GC-C; STAR; GUCY2C; GUC2C; STA receptor; DIAR6; EC 4.6.1; GCC; GUC2CEC 4.6.1.2; MUCIL |
| Gene/Protein ID (Accession) | P25092 |
| Species | Human |
| Exact Sequence | Ser24-Gln430 |
| Molecular Weight | The protein has a predicted MW of 72.8 kDa. Due to glycosylation, the protein migrates to 85-115 kDa based on Tris-Bis PAGE result. |
| Activity | Immobilized Human GUCY2C, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-GUCY2C Antibody, hFc Tag with the EC50 of 25.6ng/ml determined by ELISA. See testing image for detail. |
| Product Tag | C-hFc |
| Express System | HEK293 |
| Endotoxin | Less than 1EU per ug by the LAL method. |
Shipping, Reconstitution & Storage
| Form | Lyophilized |
| Shipping | Shipped at ambient temperature. |
| Formulation | Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| Reconstitution | Centrifuge tubes before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Stability and Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
Validation Spectrums
| Spectrums |  |
Buy Reagent | |
| ChemWhat 1412381 | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
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