Human Her2/ErbB2 Protein, Accession#: P04626, CAS#: 3202-706-1097; ChemWhat Code: 1413533
Overview
| Product Description | Recombinant Human Her2/ErbB2 Protein is expressed from HEK293 with hFc tag at the C-terminal.It contains Thr23-Thr652. |
| Biotinylated |
Specification
| Target | Her2/ErbB2 |
| Synonyms | CD340; EGFR2; ErbB2; HER2; HER-2; herstatin; MLN 19; MLN19; Neu Oncogene; NEUHER-2/neu; NGL; p185erbB2; TKR1; ERBB2; HER-2/neu; NEU; ENV; ENVW; ERVWE1; HERV-7q; HERV-W-ENV; HERV7Q; HERVW; HERVWENV |
| Gene/Protein ID (Accession) | P04626 |
| Species | Human |
| Exact Sequence | Thr23-Thr652 |
| Molecular Weight | The protein has a predicted MW of 96.1 kDa. Due to glycosylation, the protein migrates to 100-120 kDa based on Tris-Bis PAGE result. |
| Activity | Immobilized Human Her2, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-Her2 Antibody , hFc Tag with the EC50 of 0.26ug/ml determined by ELISA. See testing image for detail. |
| Product Tag | C-hFc |
| Express System | HEK293 |
| Endotoxin | Less than 1EU per ug by the LAL method. |
Shipping, Reconstitution & Storage
| Form | Lyophilized |
| Shipping | Shipped at ambient temperature. |
| Formulation | Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| Reconstitution | Centrifuge tubes before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Stability and Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
Validation Spectrums
| Spectrums |  |
Buy Reagent | |
| ChemWhat 1413533 | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
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