Human HGF Protein, Accession#: P14210, CAS#: 3202-706-386; ChemWhat Code: 1412389
Overview
| Product Description | Recombinant Human HGF Protein is expressed from Expi293 with hFc tag at the C-terminal.It contains Gln32-Ser728. |
| Biotinylated |
Specification
| Target | HGF |
| Synonyms | Hepatopoietin-A; SF; HPTA; HGF; DFNB39; F-TCF; F-TCFB; HGFB |
| Gene/Protein ID (Accession) | P14210 |
| Species | Human |
| Exact Sequence | Gln32-Ser728 |
| Molecular Weight | The HGF single chain can be processed into the active form of disulfide-linked heterodimer of α and β chain.The protein has a predicted MW of 79.6 kDa (after cleavage of the signal peptide). Due to glycosylation, the protein migrates to 60-65 kDa corresponding to α chain and β chain based on Bis-Tris PAGE result. |
| Activity | Immobilized Human HGF R, His Tag at 2μg/ml (100μl/Well) on the plate. Dose response curve for Human HGF, hFc Tag with the EC50 of 15.2ng/ml determined by ELISA. See testing image for detail. |
| Product Tag | C-hFc |
| Express System | Expi293 |
| Endotoxin | Less than 1EU per μg by the LAL method. |
Shipping, Reconstitution & Storage
| Form | Lyophilized |
| Shipping | Shipped at ambient temperature. |
| Formulation | Lyophilized from 0.22μm filtered solution in 20mM Tris, 150mM NaCl, 300mM Arginine (pH 7.5). Normally 8% trehalose is added as protectant before lyophilization. |
| Reconstitution | Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Stability and Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
Validation Spectrums
| Spectrums |  |
Buy Reagent | |
| ChemWhat 1412389 | > 95% as determined by Tris-Bis PAGE |
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