Human HGF R/c-MET Protein, Accession#: P08581, CAS#: 3202-706-912; ChemWhat Code: 1413247
Overview
| Product Description | Recombinant Human HGF R/c-MET Protein is expressed from HEK293 with His tag and Avi tag at the C-terminal.It contains Glu25-Thr932. |
| Biotinylated |
Specification
| Target | HGF R/c-MET |
| Synonyms | MET; oncogene MET; HGF R; HGF/SF receptor; AUTS9; cMET; Met (c-Met); RCCP2; SF receptor |
| Gene/Protein ID (Accession) | P08581 |
| Species | Human |
| Exact Sequence | Glu25-Thr932 |
| Molecular Weight | The mature form of HGF R is a heterodimer which can be cleaved into α and β chain. The protein has a predicted MW of 32.5 kDa (α chain) and 72.1 kDa (β chain). Due to glycosylation, the protein migrates to 45-60 kDa (α subunit) and 80-100 kDa (β subunit) based on Tris-Bis PAGE result. |
| Activity | Immobilized Human HGF R, His Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-HGF R Antibody, hFc Tag with the EC50 of 17.3ng/ml determined by ELISA (QC test). See testing image for detail. |
| Product Tag | C-His-Avi |
| Express System | HEK293 |
| Endotoxin | Less than 1EU per ug by the LAL method. |
Shipping, Reconstitution & Storage
| Form | Lyophilized |
| Shipping | Shipped at ambient temperature. |
| Formulation | Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. |
| Reconstitution | Centrifuge tubes before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Stability and Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
Validation Spectrums
| Spectrums |  |
Buy Reagent | |
| ChemWhat 1413247 | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
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