Human IGFBP-3 Protein, Accession#: AAH18962, CAS#: 3202-706-1387; ChemWhat Code: 1413885
Overview
| Product Description | Recombinant Human IGFBP-3 Protein is expressed from HEK293 with His tag at the C-terminal.It contains Gly28-Lys291. |
| Biotinylated |
Specification
| Target | IGFBP-3 |
| Synonyms | IBP3; IGFBP3; IGFBP-3; BP-53 |
| Gene/Protein ID (Accession) | AAH18962 |
| Species | Human |
| Exact Sequence | Gly28-Lys291 |
| Molecular Weight | The protein has a predicted MW of 29.8 kDa. Due to glycosylation, the protein migrates to 45-60 kDa based on Tris-Bis PAGE result. |
| Activity | Immobilized Human IGFBP-3, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human IGF-II, hFc Tag with the EC50 of 0.14ug/ml determined by ELISA (QC Test). The affinity constant of 2.32 nM as determined in SPR assay (Biacore T200). See testing image for detail. |
| Product Tag | C-His |
| Express System | HEK293 |
| Endotoxin | Less than 1EU per ug by the LAL method. |
Shipping, Reconstitution & Storage
| Form | Lyophilized |
| Shipping | Shipped at ambient temperature. |
| Formulation | Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| Reconstitution | Centrifuge tubes before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Stability and Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
Validation Spectrums
| Spectrums |  |
Buy Reagent | |
| ChemWhat 1413885 | > 95% as determined by Tris-Bis PAGE |
| No reagent supplier? | Send quick inquiry to ChemWhat |
| Want to be listed here as a reagent supplier? (Paid service) | Click here to contact ChemWhat |
Approved Manufacturers | |
| Want to be listed as an approved manufacturer (Requires approvement)? | Please download and fill out this form and send back to approved-manufacturers@chemwhat.com |
Other Suppliers | |
| Watson International Limited | Visit Watson Official Website |
Contact Us for Other Help | |
| Contact us for other information or services | Click here to contact ChemWhat |