Human IL-10 Protein, Accession#: P22301, CAS#: 3202-706-951; ChemWhat Code: 1413311
Overview
| Product Description | Recombinant Human IL-10 Protein is expressed from HEK293 without tag.It contains Ser19-Asn178. |
| Biotinylated |
Specification
| Target | IL-10 |
| Synonyms | Interleukin-10; IL-10; CSIF; IL10A; TGIF; B-TCGF; GVHDS; MGC126450; MGC126451 |
| Gene/Protein ID (Accession) | P22301 |
| Species | Human |
| Exact Sequence | Ser19-Asn178 |
| Molecular Weight | The protein has a predicted MW of 18.8 kDa same as Tris-Bis PAGE result. |
| Activity | Immobilized Human IL-10, No Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human IL-10 R alpha, His Tag with the EC50 of 21.8ng/ml determined by ELISA (QC Test). The affinity constant of 0.255 nM as determined in SPR assay (Biacore T200). See testing image for detail. |
| Product Tag | No Tag |
| Express System | HEK293 |
| Endotoxin | Less than 1EU per ug by the LAL method. |
Shipping, Reconstitution & Storage
| Form | Lyophilized |
| Shipping | Shipped at ambient temperature. |
| Formulation | Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| Reconstitution | Centrifuge tubes before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Stability and Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
Validation Spectrums
| Spectrums |  |
Buy Reagent | |
| ChemWhat 1413311 | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
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