Human IL-11 Protein, Accession#: P20809, CAS#: 3202-706-2286; ChemWhat Code: 1414771
Overview
| Product Description | Recombinant Human IL-11 Protein is expressed from HEK293 with His tag at the N-terminal.It contains Pro22-Leu199. |
| Biotinylated |
Specification
| Target | IL-11 |
| Synonyms | Interleukin-11;IL11;Adipogenesis inhibitory factor;AGIF;INN: Oprelvekin;Oprelvekin;IL-11Oprelvekin;interleukin 11;interleukin-11 |
| Gene/Protein ID (Accession) | P20809 |
| Species | Human |
| Exact Sequence | Pro22-Leu199 |
| Molecular Weight | The protein has a predicted MW of 20.24 kDa. Due to glycosylation, the protein migrates to 21-25 kDa based on Tris-Bis PAGE result. |
| Activity | Immobilized Human IL-11, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Human IL-11 R alpha, hFc Tag with the EC50 of 26.2ng/ml determined by ELISA (QC Test). The affinity constant of 1.86 nM as determined in SPR assay (Biacore T200). See testing image for detail. |
| Product Tag | N-His |
| Express System | HEK293 |
| Endotoxin | Less than 1EU per ug by the LAL method. |
Shipping, Reconstitution & Storage
| Form | Lyophilized |
| Shipping | Shipped at ambient temperature. |
| Formulation | Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| Reconstitution | Centrifuge tubes before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Stability and Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
Validation Spectrums
| Spectrums |  |
Buy Reagent | |
| ChemWhat 1414771 | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
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