Human IL-15 Protein, Accession#: P40933, CAS#: 3202-706-1295; ChemWhat Code: 1413769

Overview Specification
Shipping, Reconstitution & Storage Validation Spectrums

Overview

Product Description Recombinant Human IL-15 Protein is expressed from E.coli without tag.It contains Asn49-Ser162.
Biotinylated

Specification

Target IL-15
Synonyms L15; IL-15; IL-15MGC9721; Interleukin-15
Gene/Protein ID (Accession) P40933
Species Human
Exact Sequence Asn49-Ser162
Molecular Weight The protein has a predicted MW of 12.8 kDa same as Tris-Bis PAGE result.
Activity Immobilized Human IL-15 at 1ug/ml (100ul/Well) on the plate. Dose response curve for Human IL-15RA, His Tag with the EC50 of 22.1ng/ml determined by ELISA. The affinity constant of 5.9nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag No Tag
Express System E.coli
Endotoxin Less than 1EU per ug by the LAL method.

Shipping, Reconstitution & Storage

Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability and Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

Validation Spectrums

Spectrums

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ChemWhat 1413769> 95% as determined by Tris-Bis PAGE;> 95% as determined by SEC-HPLC
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