Human IL-18RAP Protein, Accession#: O95256, CAS#: 3202-706-1487; ChemWhat Code: 1413995
Overview
| Product Description | Recombinant Human IL-18RAP Protein is expressed from HEK293 with hFc tag at the C-terminal.It contains Phe20-Arg356. |
| Biotinylated |
Specification
| Target | IL-18RAP |
| Synonyms | ACPL; CD218b; CDw218b; IL-18R-beta; IL-18RAcP; IL-18Rbeta; IL-1R-7; IL-1R7; IL-1RAcPL; IL18RB; IL18RAP; IL-18R-bet |
| Gene/Protein ID (Accession) | O95256 |
| Species | Human |
| Exact Sequence | Phe20-Arg356 |
| Molecular Weight | The protein has a predicted MW of 65.3 kDa. Due to glycosylation, the protein migrates to 70-80 kDa based on Tris-Bis PAGE result. |
| Activity | Immobilized Human IL-18RAP, hFc Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-IL-18RAP Antibody, hFc Tag with the EC50 of 18.2ng/ml determined by ELISA. See testing image for detail. |
| Product Tag | C-hFc |
| Express System | HEK293 |
| Endotoxin | Less than 1EU per ug by the LAL method. |
Shipping, Reconstitution & Storage
| Form | Lyophilized |
| Shipping | Shipped at ambient temperature. |
| Formulation | Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. |
| Reconstitution | Centrifuge tubes before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Stability and Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
Validation Spectrums
| Spectrums |  |
Buy Reagent | |
| ChemWhat 1413995 | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
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