Human IL-1R2/IL-1 RII/CD121b Protein, Accession#: NP_004624, CAS#: 3202-706-1197; ChemWhat Code: 1413661
Overview
| Product Description | Recombinant Human IL-1R2/IL-1 RII/CD121b Protein is expressed from HEK293 with hFc tag at the C-terminal.It contains Phe14-Glu343. |
| Biotinylated |
Specification
| Target | IL-1R2/IL-1 RII/CD121b |
| Synonyms | IL-1R-2; IL-1RT-2; IL-1RT2; CDw121b; IL-1R-beta; CD121b; IL1RB; IL1R2; IL-1 RII; MGC47725 |
| Gene/Protein ID (Accession) | NP_004624 |
| Species | Human |
| Exact Sequence | Phe14-Glu343 |
| Molecular Weight | The protein has a predicted MW of 64.4 kDa. Due to glycosylation, the protein migrates to 70-80 kDa based on Tris-Bis PAGE result. |
| Activity | Immobilized Human IL-1 Beta, His Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Human IL-1R2, hFc Tag with the EC50 of 0.44ug/ml determined by ELISA (QC Test). The affinity constant of 0.88 nM as determined in SPR assay (Biacore T200). See testing image for detail. |
| Product Tag | C-hFc |
| Express System | HEK293 |
| Endotoxin | Less than 1EU per ug by the LAL method. |
Shipping, Reconstitution & Storage
| Form | Lyophilized |
| Shipping | Shipped at ambient temperature. |
| Formulation | Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. |
| Reconstitution | Centrifuge tubes before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Stability and Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
Validation Spectrums
| Spectrums |  |
Buy Reagent | |
| ChemWhat 1413661 | > 95% as determined by Tris-Bis PAGE |
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