Human IL-2 R beta&IL-2 R gamma Protein, Accession#: P14784, CAS#: 3202-706-2287; ChemWhat Code: 1414773
Overview
| Product Description | Recombinant Human IL-2 R beta&IL-2 R gamma Protein is expressed from HEK293 with hFc tag at the C-terminal.It contains Ala27-Thr240 (IL-2 R beta)&Leu23-Ala262 (IL-2 R gamma). |
| Biotinylated |
Specification
| Target | IL-2 R beta&IL-2 R gamma |
| Synonyms | IL-2RB&IL-2RG; IL-2RG; IL-2R subunit gamma; IL-2R subunit beta; IL-2RB; IL15RB; IL-2 R beta&IL-2 R gamma |
| Gene/Protein ID (Accession) | P14784 |
| Species | Human |
| Exact Sequence | Ala27-Thr240(IL-2 R beta) & Leu23-Ala262(IL-2 R gamma) |
| Molecular Weight | The protein has a predicted MW of 51.3 kDa (IL-2 R beta)&54.6 kDa (IL-2 R gamma). Due to glycosylation, the protein migrates to 65-70 kDa&80-100 kDa based on Tris-Bis PAGE result. |
| Activity | Immobilized Human IL-15RA&IL-15, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human IL-2 R beta&IL-2 R gamma, hFc Tag with the EC50 of 72.0ng/ml determined by ELISA (QC Test). The affinity constant of 0.114 nM as determined in SPR assay (Biacore T200). See testing image for detail. |
| Product Tag | C-hFc |
| Express System | HEK293 |
| Endotoxin | Less than 1EU per ug by the LAL method. |
Shipping, Reconstitution & Storage
| Form | Lyophilized |
| Shipping | Shipped at ambient temperature. |
| Formulation | Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| Reconstitution | Centrifuge tubes before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Stability and Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
Validation Spectrums
| Spectrums |  |
Buy Reagent | |
| ChemWhat 1414773 | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
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