Human IL-27 Protein, Accession#: Q14213, CAS#: 3202-706-1143; ChemWhat Code: 1413589
Overview
| Product Description | Recombinant Human IL-27 Protein is expressed from CHO with His tag at the C-terminal.It contains Arg21-Lys229(IL-27B)&Phe29-Pro243(IL-27A). |
| Biotinylated |
Specification
| Target | IL-27 |
| Synonyms | |
| Gene/Protein ID (Accession) | Q14213 |
| Species | Human |
| Exact Sequence | Arg21-Lys229(IL-27B) & Phe29-Pro243(IL-27A) |
| Molecular Weight | The protein has a predicted MW of 50.6 kDa. Due to glycosylation, the protein migrates to 54-64 kDa based on Tris-Bis PAGE result. |
| Activity | Immobilized Human IL-27 RA, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human IL-27, His Tag with the EC50 of 22.7ng/ml determined by ELISA (QC Test). The affinity constant of 3.58 nM as determined in SPR assay (Biacore T200). See testing image for detail. |
| Product Tag | C-His |
| Express System | CHO |
| Endotoxin | Less than 1EU per ug by the LAL method. |
Shipping, Reconstitution & Storage
| Form | Lyophilized |
| Shipping | Shipped at ambient temperature. |
| Formulation | Lyophilized from 0.22um filtered solution in PBS, 200mM L-arginine (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| Reconstitution | Centrifuge tubes before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Stability and Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
Validation Spectrums
| Spectrums |  |
Buy Reagent | |
| ChemWhat 1413589 | > 95% as determined by Tris-Bis PAGE |
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