Human IL-31 Protein, Accession#: Q6EBC2, CAS#: 3202-706-1854; ChemWhat Code: 1414351
Overview
| Product Description | Recombinant Human IL-31 Protein is expressed from HEK293 with His tag at the N-terminal.It contains Ser24-Thr164. |
| Biotinylated |
Specification
| Target | IL-31 |
| Synonyms | Interleukin-31; IL-31;IL31 |
| Gene/Protein ID (Accession) | Q6EBC2 |
| Species | Human |
| Exact Sequence | Ser24-Thr164 |
| Molecular Weight | The protein has a predicted MW of 16.9 kDa. Due to glycosylation, the protein migrates to 20-25 kDa based on Tris-Bis PAGE result. |
| Activity | Immobilized Human IL-31, His Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Human IL-31 RA, hFc Tag with the EC50 of 1.15ug/ml determined by ELISA (QC Test). The affinity constant of 1.5nM as determined in SPR assay (Biacore T200). See testing image for detail. |
| Product Tag | N-His |
| Express System | HEK293 |
| Endotoxin | Less than 1EU per ug by the LAL method. |
Shipping, Reconstitution & Storage
| Form | Lyophilized |
| Shipping | Shipped at ambient temperature. |
| Formulation | Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. |
| Reconstitution | Centrifuge tubes before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Stability and Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
Validation Spectrums
| Spectrums | ![]() |
Buy Reagent | |
| ChemWhat 1414351 | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
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