Human IL-31 RA Protein, Accession#: Q8NI17, CAS#: 3202-706-1506; ChemWhat Code: 1414015
Overview
| Product Description | Recombinant Human IL-31 RA Protein is expressed from HEK293 with hFc tag at the C-terminal.It contains Ala20-Glu519. |
| Biotinylated |
Specification
| Target | IL-31 RA |
| Synonyms | IL-31RA; IL-31R-alpha; Gp130-like receptor; CRL3; GPL; ZcytoR17; GLM-R; CRL3Glmr; GLM-RMGC125346; PRO21384 |
| Gene/Protein ID (Accession) | Q8NI17 |
| Species | Human |
| Exact Sequence | Ala20-Glu519 |
| Molecular Weight | The protein has a predicted MW of 83.8 kDa. Due to glycosylation, the protein migrates to 110-135 kDa based on Tris-Bis PAGE result. |
| Activity | Immobilized Human IL-31 RA, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-IL-31 RA Antibody, hFc Tag with the EC50 of 16.9ng/ml determined by ELISA (QC Test). The affinity constant of 1.5nM as determined in SPR assay (Biacore T200). See testing image for detail. |
| Product Tag | C-hFc |
| Express System | HEK293 |
| Endotoxin | Less than 1EU per ug by the LAL method. |
Shipping, Reconstitution & Storage
| Form | Lyophilized |
| Shipping | Shipped at ambient temperature. |
| Formulation | Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. |
| Reconstitution | Centrifuge tubes before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Stability and Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
Validation Spectrums
| Spectrums |  |
Buy Reagent | |
| ChemWhat 1414015 | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
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