Human IL-33 Protein, Accession#: NP_254274, CAS#: 3202-706-1355; ChemWhat Code: 1413849

Overview Specification
Shipping, Reconstitution & Storage Validation Spectrums

Overview

Product Description Recombinant Human IL-33 Protein is expressed from E.coli without tag.It contains Ser112-Thr270.
Biotinylated

Specification

Target IL-33
Synonyms C9orf26 Z; DVS27; IL1F11; NF-HEV; NFEHEV; IL33; DV27; C9ORF26; RP11-575C20.2
Gene/Protein ID (Accession) NP_254274
Species Human
Exact Sequence Ser112-Thr270
Molecular Weight The protein has a predicted MW of 18 kDa same as Tris-Bis PAGE result.
Activity Immobilized Human IL-33, No Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human IL-1RL1, His Tag with the EC50 of 6.1ng/ml determined by ELISA. See testing image for detail.
Product Tag No Tag
Express System E.coli
Endotoxin Less than 1EU per ug by the LAL method.

Shipping, Reconstitution & Storage

Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability and Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

Validation Spectrums

Spectrums

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ChemWhat 1413849> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
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