Human IL-6 Protein, Accession#: P05231, CAS#: 3202-706-969; ChemWhat Code: 1413343
Overview
| Product Description | Recombinant Human IL-6 is expressed from E.coli without tag.It contains Val30-Met212. |
| Biotinylated |
Specification
| Target | IL-6 |
| Synonyms | IFN-beta-2; IL6; IL-6; BSF-2; CDF; MGI-2A; Interleukin-6; HSF; IFNB2; HGF |
| Gene/Protein ID (Accession) | P05231 |
| Species | Human |
| Exact Sequence | Val30-Met212 |
| Molecular Weight | The protein has a predicted 20.8 kDa same as Tris-Bis PAGE result. |
| Activity | Immobilized Human IL-6 at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Human IL-6 R alpha, hFc Tag with the EC50 48.7ng/ml determined by ELISA. The affinity constant of 0.22 nM as determined in SPR assay (Biacore T200). See testing image for detail. |
| Product Tag | No Tag |
| Express System | E.coli |
| Endotoxin | Less than 1EU per ug by the LAL method. |
Shipping, Reconstitution & Storage
| Form | Lyophilized |
| Shipping | Shipped at ambient temperature. |
| Formulation | Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. |
| Reconstitution | Centrifuge tubes before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Stability and Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
Validation Spectrums
| Spectrums |  |
Buy Reagent | |
| ChemWhat 1413343 | > 95% as determined by Tris-Bis PAGE;> 90% as determined by HPLC |
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