Human LRP-5 Protein, Accession#: O75197, CAS#: 3202-706-753; ChemWhat Code: 1412977
Overview
| Product Description | Recombinant Human LRP-5 Protein is expressed from HEK293 with mFc (IgG1) tag at the C-terminal.It contains Glu644-Gln1263. |
| Biotinylated |
Specification
| Target | LRP-5 |
| Synonyms | LRP-5; LRP-7; LR3; BMND1; BMND1OPTA1; EVR1; EVR4; HBM; LR3VBCH2; LRP5; LRP7; OPPG; OPS; OPTA1; VBCH2 |
| Gene/Protein ID (Accession) | O75197 |
| Species | Human |
| Exact Sequence | Glu644-Gln1263 |
| Molecular Weight | The protein has a predicted MW of 96 kDa. Due to glycosylation, the protein migrates to 100-115 kDa based on Tris-Bis PAGE result. |
| Activity | Immobilized Human DKK1, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human LRP-5, mFc Tag with the EC50 of 0.11ug/ml determined by ELISA. See testing image for detail. |
| Product Tag | C-mFc |
| Express System | HEK293 |
| Endotoxin | Less than 1EU per ug by the LAL method. |
Shipping, Reconstitution & Storage
| Form | Lyophilized |
| Shipping | Shipped at ambient temperature. |
| Formulation | Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| Reconstitution | Centrifuge tubes before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Stability and Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
Validation Spectrums
| Spectrums |  |
Buy Reagent | |
| ChemWhat 1412977 | > 95% as determined by Tris-Bis PAGE;> 90% as determined by HPLC |
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Other Suppliers | |
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