Human NGAL/Lipocalin-2 Protein, Accession#: P80188, CAS#: 3202-706-456; ChemWhat Code: 1412517
Overview
| Product Description | Recombinant Human NGAL/Lipocalin-2 Protein is expressed from HEK293 with hFc tag at the C-terminal.It contains Gln21-Gly198. |
| Biotinylated |
Specification
| Target | NGAL/Lipocalin-2 |
| Synonyms | NGAL; Lipocalin-2; Oncogene 24p3; p25; Siderocalin LCN2; MSFI |
| Gene/Protein ID (Accession) | P80188 |
| Species | Human |
| Exact Sequence | Gln21-Gly198 |
| Molecular Weight | The protein has a predicted MW of 47.2 kDa. Due to glycosylation, the protein migrates to 48-52 kDa based on Tris-Bis PAGE result. |
| Activity | Immobilized Human NGAL, hFc Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Anti-NGAL Antibody, hFc Tag with the EC50 19.7ng/ml determined by ELISA. See testing image for detail. |
| Product Tag | C-hFc |
| Express System | HEK293 |
| Endotoxin | Less than 1EU per ug by the LAL method. |
Shipping, Reconstitution & Storage
| Form | Lyophilized |
| Shipping | Shipped at ambient temperature. |
| Formulation | Lyophilized from 0.22um filtered solution in 50mM MES,150mM NaCl (pH 6.5). Normally 8% trehalose is added as protectant before lyophilization. |
| Reconstitution | Centrifuge tubes before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in 50mM MES,150mM NaCl (pH 6.5). |
| Stability and Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
Validation Spectrums
| Spectrums |  |
Buy Reagent | |
| ChemWhat 1412517 | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
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