Human NKG2D/CD314 Protein, Accession#: P26718, CAS#: 3202-706-1512; ChemWhat Code: 1414023
Overview
| Product Description | Recombinant Human NKG2D/CD314 Protein is expressed from HEK293 with hFc tag and Flag tag at the N-terminal.It contains Phe78-Val216. |
| Biotinylated |
Specification
| Target | NKG2D/CD314 |
| Synonyms | CD314; D12S2489E; KLR; NKG2-D; NKG2D |
| Gene/Protein ID (Accession) | P26718 |
| Species | Human |
| Exact Sequence | Phe78-Val216 |
| Molecular Weight | The protein has a predicted MW of 43.4 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on Tris-Bis PAGE result. |
| Activity | Immobilized Human ULBP-2, His Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Human NKG2D, hFc Tag with the EC50 of 2.8ug/ml determined by ELISA (QC Test). See testing image for detail. |
| Product Tag | N-hFc-Flag |
| Express System | HEK293 |
| Endotoxin | Less than 1EU per ug by the LAL method. |
Shipping, Reconstitution & Storage
| Form | Lyophilized |
| Shipping | Shipped at ambient temperature. |
| Formulation | Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. |
| Reconstitution | Centrifuge tubes before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Stability and Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
Validation Spectrums
| Spectrums |  |
Buy Reagent | |
| ChemWhat 1414023 | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
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