Human Periostin/OSF-2 Protein, Accession#: Q15063, CAS#: 3202-706-459; ChemWhat Code: 1412521
Overview
| Product Description | Recombinant Human Periostin/OSF-2 Protein is expressed from HEK293 with His tag at the C-terminal.It contains Asn22-Gln836. |
| Biotinylated |
Specification
| Target | Periostin/OSF-2 |
| Synonyms | Periostin; PN; OSF-2; POSTN; OSF2; Fasciclin I-like; PDLPOSTN; PNRP11-412K4.1; TRIF52 |
| Gene/Protein ID (Accession) | Q15063 |
| Species | Human |
| Exact Sequence | Asn22-Gln836 |
| Molecular Weight | The protein has a predicted MW of 92.1 kDa. Due to glycosylation, the protein migrates to 93-100 kDa based on Tris-Bis PAGE result. |
| Activity | Immobilized Human Periostin, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-Periostin Antibody, hFc Tag with the EC50 of 18.5ng/ml determined by ELISA. See testing image for detail. |
| Product Tag | C-His |
| Express System | HEK293 |
| Endotoxin | Less than 1EU per ug by the LAL method. |
Shipping, Reconstitution & Storage
| Form | Lyophilized |
| Shipping | Shipped at ambient temperature. |
| Formulation | Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| Reconstitution | Centrifuge tubes before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Stability and Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
Validation Spectrums
| Spectrums |  |
Buy Reagent | |
| ChemWhat 1412521 | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
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