Human PRAME (HLA-A*02:01) Protein, Accession#: P79603, CAS#: 3202-706-646; ChemWhat Code: 1412804
Overview
| Product Description | Recombinant Human PRAME (HLA-A*02:01) Protein is expressed from HEK293 with His tag and Avi tag at the C-terminal.It contains Gly25-Thr305(HLA-A*02:01), Ile21-Met119(B2M) and ALYVDSLFFL peptide. |
| Biotinylated |
Specification
| Target | PRAME (HLA-A*02:01) |
| Synonyms | |
| Gene/Protein ID (Accession) | P79603 |
| Species | Human |
| Exact Sequence | Gly25-Thr305(HLA-A*02:01), Ile21-Met119(B2M) and ALYVDSLFFL peptide |
| Molecular Weight | The protein has a predicted MW of 50.60 kDa. Due to glycosylation, the protein migrates to 53-63 kDa based on Tris-Bis PAGE result. |
| Activity | Immobilized Human PRAME (HLA-A*02:01) , His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Anti-PRAME (HLA-A*02:01) Antibody, hFc Tag with the EC50 of 54.2ng/ml determined by ELISA. See testing image for detail. |
| Product Tag | C-His-Avi |
| Express System | HEK293 |
| Endotoxin | Less than 1EU per ug by the LAL method. |
Shipping, Reconstitution & Storage
| Form | Liquid |
| Shipping | Shipped with blue ice. |
| Formulation | Supplied as 0.22um filtered solution in PBS, 100mM Arginine (pH 7.4). |
| Reconstitution | |
| Stability and Storage | Valid for 12 months from date of receipt when stored at -80°C.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please avoid repeated freeze-thaw cycles. |
Validation Spectrums
| Spectrums |  |
Buy Reagent | |
| ChemWhat 1412804 | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
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