Human PRLR Protein, Accession#: P16471, CAS#: 3202-706-1321; ChemWhat Code: 1413803

Overview Specification
Shipping, Reconstitution & Storage Validation Spectrums

Overview

Product Description Recombinant Human PRLR Protein is expressed from HEK293 with His tag at the C-terminal.It contains Gln25-Asp234.
Biotinylated

Specification

Target PRLR
Synonyms PRL-R; HPRL; MFAB; Prolactin R
Gene/Protein ID (Accession) P16471
Species Human
Exact Sequence Gln25-Asp234
Molecular Weight The protein has a predicted MW of 25.5 kDa. Due to glycosylation, the protein migrates to 35-40 kDa based on Tris-Bis PAGE result.
Activity Immobilized Human PRLR, His Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Anti-PRLR Antibody, hFc Tag with the EC50 of 12.3ng/ml determined by ELISA (QC Test). The affinity constant of 0.96nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag C-His
Express System HEK293
Endotoxin Less than 1EU per ug by the LAL method.

Shipping, Reconstitution & Storage

Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in 50mM Tris, 150nM Nacl (pH 7.5). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability and Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

Validation Spectrums

Spectrums

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ChemWhat 1413803> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
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