Human R spondin 1/RSPO1 Protein, Accession#: Q2MKA7, CAS#: 3202-706-474; ChemWhat Code: 1412550
Overview
| Product Description | Recombinant Human R spondin 1/RSPO1 Protein is expressed from CHO with His tag at the C-terminal.It contains Arg31-Ala263. |
| Biotinylated |
Specification
| Target | R spondin 1/RSPO1 |
| Synonyms | R-spondin-1; RSPO1; CRISTIN3; FLJ40906; RP11-566C13.1; RSPO |
| Gene/Protein ID (Accession) | Q2MKA7 |
| Species | Human |
| Exact Sequence | Arg31-Ala263 |
| Molecular Weight | The protein has a predicted MW of 27.3 kDa. Due to glycosylation, the protein migrates to 38-42 kDa based on Tris-Bis PAGE result. |
| Activity | Immobilized Human R spondin 1, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-R spondin 1 Antibody, hFc Tag with the EC50 of 7.1ng/ml determined by ELISA (QC Test). See testing image for detail. |
| Product Tag | C-His |
| Express System | CHO |
| Endotoxin | Less than 1EU per ug by the LAL method. |
Shipping, Reconstitution & Storage
| Form | Lyophilized |
| Shipping | Shipped at ambient temperature. |
| Formulation | Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. |
| Reconstitution | Centrifuge tubes before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Stability and Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
Validation Spectrums
| Spectrums |  |
Buy Reagent | |
| ChemWhat 1412550 | > 95% as determined by Tris-Bis PAGE |
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