Human Siglec-15/CD33L3 Protein, Accession#: Q6ZMC9, CAS#: 3202-706-757; ChemWhat Code: 1412985
Overview
| Product Description | Recombinant Human Siglec-15/CD33L3 Protein is expressed from HEK293 with His tag and Avi tag at the C-terminal.It contains Phe20-Thr263. |
| Biotinylated |
Specification
| Target | Siglec-15/CD33L3 |
| Synonyms | CD33 molecule-like 3; CD33L3; HsT1361; Siglec15; CD33 antigen-like 3; SIGLEC-15 |
| Gene/Protein ID (Accession) | Q6ZMC9 |
| Species | Human |
| Exact Sequence | Phe20-Thr263 |
| Molecular Weight | The protein has a predicted MW of 29.5 kDa. Due to glycosylation, the protein migrates to 30-38 kDa based on Tris-Bis PAGE result. |
| Activity | Immobilized Human Siglec-15, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-Siglec-15 Antibody, hFc Tag with the EC50 of 4.6ng/ml determined by ELISA. See testing image for detail. |
| Product Tag | C-His-Avi |
| Express System | HEK293 |
| Endotoxin | Less than 1EU per ug by the LAL method. |
Shipping, Reconstitution & Storage
| Form | Lyophilized |
| Shipping | Shipped at ambient temperature. |
| Formulation | Lyophilized from 0.22um filtered solution in 20mM NaAC (pH 5.0). Normally 8% trehalose is added as protectant before lyophilization. |
| Reconstitution | Centrifuge tubes before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in 20mM NaAC (pH 5.0). |
| Stability and Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
Validation Spectrums
| Spectrums |  |
Buy Reagent | |
| ChemWhat 1412985 | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
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