Human SIRP alpha V4 Protein, Accession#: P78324, CAS#: 3202-706-1135; ChemWhat Code: 1413579
Overview
| Product Description | Recombinant Human SIRP alpha V4 Protein is expressed from HEK293 with His tag and Avi tag at the C-terminal.It contains Glu31-Arg370(E33G, L44S, T50S, T52I, R54H). |
| Biotinylated |
Specification
| Target | SIRP alpha V4 |
| Synonyms | CD172a; BIT; MFR; MYD1; MYD-1; P84; PTPNS1; SHP substrate 1; SHPS1; SHPS-1; SHPS1CD172A; SIRP alpha; SIRPA; Sirp-alpha-1; SIRPalpha2; Sirp-alpha-2; Sirp-alpha-3 |
| Gene/Protein ID (Accession) | P78324 |
| Species | Human |
| Exact Sequence | Glu31-Arg370(E33G, L44S, T50S, T52I, R54H) |
| Molecular Weight | The protein has a predicted MW of 39.8 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on Tris-Bis PAGE result. |
| Activity | Immobilized Human SIRP alpha V4, His Tag at 1ug/ml (100ul/Well). Dose response curve for Human CD47, hFc Tag with the EC50 of 44.3ng/ml determined by ELISA. See testing image for detail. |
| Product Tag | C-His-Avi |
| Express System | HEK293 |
| Endotoxin | Less than 1EU per ug by the LAL method. |
Shipping, Reconstitution & Storage
| Form | Lyophilized |
| Shipping | Shipped at ambient temperature. |
| Formulation | Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. |
| Reconstitution | Centrifuge tubes before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Stability and Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
Validation Spectrums
| Spectrums |  |
Buy Reagent | |
| ChemWhat 1413579 | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
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