Human TNF alpha Protein, Accession#: P01375, CAS#: 3202-706-1505; ChemWhat Code: 1414013
Overview
| Product Description | Recombinant Human TNF alpha Protein is expressed from HEK293 with His tag and Avi tag at the C-terminal.It contains Val77-Leu233. |
| Biotinylated |
Specification
| Target | TNF alpha |
| Synonyms | APC1 protein; Cachectin; DIF; TNF; TNFalpha; TNFATNF; TNFSF1A; TNFSF2; TNFA; TNFα; DIF; TNFSF2 |
| Gene/Protein ID (Accession) | P01375 |
| Species | Human |
| Exact Sequence | Val77-Leu233 |
| Molecular Weight | The protein has a predicted MW of 20.3 kDa. Due to glycosylation, the protein migrates to 21-25 kDa based on Tris-Bis PAGE result. |
| Activity | Immobilized Human TNF alpha, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human TNFR2, mFc Tag with the EC50 of 38.5ng/ml determined by ELISA. The affinity constant of 1.62 nM as determined in SPR assay (Biacore T200). See testing image for detail. |
| Product Tag | C-His-Avi |
| Express System | HEK293 |
| Endotoxin | Less than 1EU per ug by the LAL method. |
Shipping, Reconstitution & Storage
| Form | Lyophilized |
| Shipping | Shipped at ambient temperature. |
| Formulation | Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. |
| Reconstitution | Centrifuge tubes before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Stability and Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
Validation Spectrums
| Spectrums |  |
Buy Reagent | |
| ChemWhat 1414013 | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
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Other Suppliers | |
| Watson International Limited | Visit Watson Official Website |
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