Human ULBP-1 Protein, Accession#: Q9BZM6, CAS#: 3202-706-876; ChemWhat Code: 1413195
Overview
| Product Description | Recombinant Human ULBP-1 Protein is expressed from HEK293 with His tag and Avi tag at the C-terminal.It contains Gly26-Pro215. |
| Biotinylated |
Specification
| Target | ULBP-1 |
| Synonyms | ALCAN-beta; NKG2D ligand 1; N2DL-1; NKG2DL1; MULT-1; NKG2DL; AET1I; ULBP1; ULBP-1 |
| Gene/Protein ID (Accession) | Q9BZM6 |
| Species | Human |
| Exact Sequence | Gly26-Pro215 |
| Molecular Weight | The protein has a predicted MW of 25.2 kDa. Due to glycosylation, the protein migrates to 28-35 kDa based on Tris-Bis PAGE result. |
| Activity | Immobilized Human ULBP-1, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human NKG2D, hFc Tag with the EC50 of 0.81ug/ml determined by ELISA. See testing image for detail. |
| Product Tag | C-His-Avi |
| Express System | HEK293 |
| Endotoxin | Less than 1EU per ug by the LAL method. |
Shipping, Reconstitution & Storage
| Form | Lyophilized |
| Shipping | Shipped at ambient temperature. |
| Formulation | Lyophilized from 0.22um filtered solution in 20mM PB, 0.5M NaCl, 0.1M L-arginine (pH 8.0). Normally 8% trehalose is added as protectant before lyophilization. |
| Reconstitution | Centrifuge tubes before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Stability and Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
Validation Spectrums
| Spectrums |  |
Buy Reagent | |
| ChemWhat 1413195 | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
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