Human VEGF R3/FLT4 Protein, Accession#: P35916, CAS#: 3202-706-759; ChemWhat Code: 1412989
Overview
| Product Description | Recombinant Human VEGF R3/FLT4 Protein is expressed from HEK293 with hFc tag at the C-terminal.It contains Tyr25-Ile776. |
| Biotinylated |
Specification
| Target | VEGF R3/FLT4 |
| Synonyms | VEGFR-3; FLT-4; LMPH1A; PCLFLT41; VEGFR3; FLT-41; PCL |
| Gene/Protein ID (Accession) | P35916 |
| Species | Human |
| Exact Sequence | Tyr25-Ile776 |
| Molecular Weight | The protein has a predicted MW of 111.3 kDa. Due to glycosylation, the protein migrates to 135-155 kDa based on Tris-Bis PAGE result. |
| Activity | Immobilized Human VEGF-C, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human VEGF R3, hFc Tag with the EC50 of 10.5ng/ml determined by ELISA. See testing image for detail. |
| Product Tag | C-hFc |
| Express System | HEK293 |
| Endotoxin | Less than 1EU per ug by the LAL method. |
Shipping, Reconstitution & Storage
| Form | Lyophilized |
| Shipping | Shipped at ambient temperature. |
| Formulation | Lyophilized from 0.22um filtered solution in 50mM Tris, 150mM NaCl, 100mM Glycine (pH 7.5). Normally 5% trehalose is added as protectant before lyophilization. |
| Reconstitution | Centrifuge tubes before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Stability and Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
Validation Spectrums
| Spectrums |  |
Buy Reagent | |
| ChemWhat 1412989 | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
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