isoleucine N-monooxygenase EC#: 1.14.14.39; ChemWhat Code: 1375547

Product Name isoleucine N-monooxygenase
Example Structure Example Structure of isoleucine N-monooxygenase EC#: 1.14.14.39
Synonyms CYP79D1, CYP79D2, CYP79D3, CYP79D4, valine N-monooxygenase
EC Number 1.14.14.39
CAS Registry Number
Comments This cytochrome P-450 (heme-thiolate) enzyme, found in plants, catalyses two successive N-hydroxylations of L-isoleucine, the committed step in the biosynthesis of the cyanogenic glucoside lotaustralin. The product of the two hydroxylations, N,N-dihydroxy-L-isoleucine, is labile and undergoes dehydration followed by decarboxylation, producing the oxime. It is still not known whether the decarboxylation is spontaneous or catalysed by the enzyme. The enzyme can also accept L-valine, but with a lower activity. cf. EC 1.14.14.38, valine N-monooxygenase. Formerly EC 1.4.13.117.
Cofactor heme-thiolate
History
Reactions L-isoleucine + 2 O(2) + 2 [reduced NADPH–hemoprotein reductase] = (1E,2S)-2-methylbutanal oxime + 2 [oxidized NADPH–hemoprotein reductase] + CO(2) + 3 H(2)O.

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