Requires Mg2+?for maximal activity. The enzyme from mammals is a bifunctional enzyme: the N-terminal domain exhibits lipid-phosphate-phosphatase activity and the C-terminal domain has the activity of EC?3.3.2.10, soluble epoxide hydrolase (sEH) [1]. The best substrates for this enzyme are 10-hydroxy-9-(phosphonooxy)octadecanoates, with the?threo- form being a better substrate than the?erythro- form [1]. The phosphatase activity is not found in plant sEH or in EC?3.3.2.9, microsomal epoxide hydrolase, from mammals [1].
