The substrate group is a substituent on N-4 of an asparagine residue in the glycoprotein. The mannose residue at the non-reducing end of the sequence may carry further ¦Á-D-mannosyl groups on O-3 or O-6, but such a substituent on O-3 of the ¦Â-D-mannosyl group prevents the action of the enzyme. The enzyme was obtained from the lily,?Lilium longiflorum.
Cofactor
History
Reactions
Hydrolysis of the alpha-D-mannosyl-(1->6)-beta-D-mannosyl-(1->4)-N- acetyl-beta-D-glucosaminyl-(1->4)-N-acetyl-beta-D-glucosaminyl sequence of glycoprotein to alpha-D-mannosyl-(1->6)-D-mannose and N-acetyl-beta-D- glucosaminyl-(1->4)-N-acetyl-beta-D-glucosaminyl sequences.
