Mouse ARTN Protein, Accession#: Q9Z0L2, CAS#: 3202-706-1367; ChemWhat Code: 1413861

Overview Specification
Shipping, Reconstitution & Storage Validation Spectrums

Overview

Product Description Recombinant Mouse ARTN Protein is expressed from HEK293 with hFc tag at the N-terminal.It contains Ala112-Gly224.
Biotinylated

Specification

Target ARTN
Synonyms Artemin; Enovin; Neublastin; ARTNE; EVN; ARTN; NBN
Gene/Protein ID (Accession) Q9Z0L2
Species Mouse
Exact Sequence Ala112-Gly224
Molecular Weight The protein has a predicted MW of 39.4 kDa. Due to glycosylation, the protein migrates to 65-80 kDa based on Tris-Bis PAGE result.
Activity Immobilized Mouse GFRA3, His Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Mouse ARTN, hFc Tag with the EC50 of 0.12ug/ml determined by ELISA. See testing image for detail.
Product Tag N-hFc
Express System HEK293
Endotoxin Less than 1EU per ug by the LAL method.

Shipping, Reconstitution & Storage

Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in 50mM Glycine,150mM NaCl (pH 3.2). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in 50mM Glycine,150mM NaCl (pH 3.2).
Stability and Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

Validation Spectrums

Spectrums

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ChemWhat 1413861> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
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