Mouse CD27 Ligand/CD70 Protein, Accession#: O55237, CAS#: 3202-706-1635; ChemWhat Code: 1414147
Overview
| Product Description | Recombinant Mouse CD27 Ligand/CD70 Protein is expressed from HEK293 with hFc tag at the N-terminal.It contains Ser45-Pro195. |
| Biotinylated |
Specification
| Target | CD27 Ligand/CD70 |
| Synonyms | CD70 molecule; CD70; TNFSF7; CD27 Ligand; CD27-L; CD27LG; Ki-24 antigen; TNFSF7G |
| Gene/Protein ID (Accession) | O55237 |
| Species | Mouse |
| Exact Sequence | Ser45-Pro195 |
| Molecular Weight | The protein has a predicted MW of 43.41 kDa. Due to glycosylation, the protein migrates to 55-60 kDa based on Tris-Bis PAGE result. |
| Activity | Immobilized Mouse CD27, mFc Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Mouse CD27 Ligand, hFc Tag with the EC50 of 35.5ng/ml determined by ELISA. See testing image for detail. |
| Product Tag | N-hFc |
| Express System | HEK293 |
| Endotoxin | Less than 1EU per ug by the LAL method. |
Shipping, Reconstitution & Storage
| Form | Lyophilized |
| Shipping | Shipped at ambient temperature. |
| Formulation | Lyophilized from 0.22um filtered solution in PBS (pH 6.2). Normally 8% trehalose is added as protectant before lyophilization. |
| Reconstitution | Centrifuge tubes before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Stability and Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
Validation Spectrums
| Spectrums |  |
Buy Reagent | |
| ChemWhat 1414147 | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
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