Mouse R spondin 1/RSPO1 Protein, Accession#: Q9Z132, CAS#: 3202-706-475; ChemWhat Code: 1412552

Overview Specification
Shipping, Reconstitution & Storage Validation Spectrums

Overview

Product Description Recombinant Mouse R spondin 1/RSPO1 Protein is expressed from HEK293 with His tag at the C-terminal.It contains Ser21-Gln265.
Biotinylated

Specification

Target R spondin 1/RSPO1
Synonyms R-spondin-1; RSPO1; CRISTIN3; FLJ40906; RP11-566C13.1; RSPO
Gene/Protein ID (Accession) Q9Z132
Species Mouse
Exact Sequence Ser21-Gln265
Molecular Weight The protein has a predicted MW of 28.3 kDa. Due to glycosylation, the protein migrates to 45-48 kDa based on Tris-Bis PAGE result.
Activity Immobilized Mouse R spondin 1, His Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Mouse RNF43, hFc Tag with the EC50 of 0.40ug/ml determined by ELISA. See testing image for detail.
Product Tag C-His
Express System HEK293
Endotoxin Less than 1EU per ug by the LAL method.

Shipping, Reconstitution & Storage

Form Liquid
Shipping Shipped with blue ice.
Formulation Supplied as 0.22um filtered solution in 20mM MES, 150mM NaCl, 200mM Arginine (pH 5.5).
Reconstitution
Stability and Storage Valid for 12 months from date of receipt when stored at -80°C.; Recommend to aliquot the protein into smaller quantities for optimal storage. Please avoid repeated freeze-thaw cycles.

Validation Spectrums

Spectrums

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ChemWhat 1412552> 95% as determined by Tris-Bis PAGE
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