The enzyme that is found in actinomycetes is involved in the detoxification of oxidizing agents and electrophilic antibiotics. The enzyme has low activity with 1-O-(2-acetamido-2-deoxy-¦Á-D-glucopyranosyl)-1D-myo-inositol as substrate (cf. EC?3.5.1.103,?N-acetyl-1-D-myo-inositol-2-amino-2-deoxy-¦Á-D-glucopyranoside deacetylase) [2].
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A mycothiol S-conjugate + H(2)O = an N-acetyl L-cysteine-S-conjugate + 1-O-(2-amino-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol.