AA3, AAIII, Acy3, aminoacylase 3, aminoacylase III
EC Number
3.5.1.114
CAS Registry Number
Comments
This enzyme is found in animals and is involved in the hydrolysis of?N-acylated or?N-acetylated amino acids (except?L-aspartate). It preferentially deacetylates?N¦Á-acetylated aromatic amino acids and mercapturic acids (S-conjugates of?N-acetyl-L-cysteine) that are usually not deacetylated by EC?3.5.1.14,?N-acyl-aliphatic-L-amino acid amidohydrolase. The enzyme is significantly activated by Co2+?and Ni2+?[3]. Some bacterial aminoacylases demonstrate substrate specificity for both EC?3.5.1.14?and EC?3.5.1.114.?cf. EC?3.5.1.14,?N-acyl-aliphatic-L-amino acid amidohydrolase and EC?3.5.1.15, aspartoacylase.
Cofactor
Zn2+
History
Reactions
(1) An N-acyl-aromatic-L-amino acid + H(2)O = an aromatic-L-amino acid + a carboxylate. (2) An N-acetyl-L-cysteine-S-conjugate + H(2)O = an L-cysteine-S- conjugate + acetate.
