This enzyme, along with EC?3.5.1.87?(N-carbamoyl-L-amino-acid hydrolase), EC?5.1.99.5?(hydantoin racemase) and hydantoinase, forms part of the reaction cascade known as the “hydantoinase process”, which allows the total conversion of?D,L-5-monosubstituted hydantoins into optically pure?D- or?L-amino acids [2]. It has strict stereospecificity for?N-carbamoyl-D-amino acids and does not act upon the corresponding?L-amino acids or?N-formyl amino acids,?N-carbamoyl-sarcosine, -citrulline, -allantoin and -ureidopropionate, which are substrates for other amidohydrolases.
