This lysosomal enzyme acts best on palmitoyl ethanolamide, with lower activity on other?N-(long-chain-acyl)ethanolamines. It is only active at acidic pH. Unlike EC?3.5.1.99, fatty acid amide hydrolase, it does not act on primary amides such as oleamide, and has only a marginal activity with anandamide. The enzyme is translated as an inactive proenzyme, followed by autocatalytic cleavage into two subunits that reassociate to form an active heterodimeric complex.
Cofactor
History
Reactions
N-(long-chain-acyl)ethanolamine + H(2)O = a long-chain carboxylate + ethanolamine.