The enzyme is involved in the synthesis of N6-threonylcarbamoyladenosine37 in tRNAs, which is found in tRNAs with the anticodon NNU, i.e. tRNAIle, tRNAThr, tRNAAsn, tRNALys, tRNASer and tRNAArg [3].
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L-threonylcarbamoyladenylate + adenine(37) in tRNA = Amp + N(6)-L- threonylcarbamoyladenine(37) in tRNA.